My longest on my home layout is 56 cars IIRC.
Three old Atlas GP-35's pulling.
The layout is an old Atlas HO-35 converted to N scale, so it's got 19" radii and2% grade.
Mix of MT, Accumate & McHenry couplers
There was about a foot of distance between the front engine and caboose.
I supose if Kiz' ever comes north with his 8 dozen orecars, We could see a larger car count!